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Silencing of FKBP5 blunts dexamethasone (DEX)-regulated expression of glucocorticoid response element-containing genes in primary leiomyoma cell cultures. qPCR analysis of mRNA levels for DEX-upregulated genes TSC22D 3 , ADH1B, IL1R1 (A), and DEX-downregulated genes GREM1, IGFBP1, <t>AMIGO2</t> (B) in cultured leiomyoma cells transfected with either control or FKBP5 siRNA for 48 hours and then treated with either vehicle (Cont) or DEX for 24 hours. Bars represent mean ± standard error of the mean, n = 4.
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a Expression of <t>AMIGO2,</t> STC2, INHBA, DKK1, VEGFC, SPOCK1, MT1E, and FOXD1 in scRNA-seq UMAP plots. b Bar plot showing cell type proportions under LRS subtypes from scRNA-seq data. c AMIGO2, ranked highly for prognostic importance, was selected for further analysis. d AMIGO2 expression is upregulated in most tumor tissues across various cancers in TCGA pan-cancer dataset. e Evaluation of AMIGO2’s prognostic value (OS) in TCGA pan-cancer dataset. f Correlation analysis between AMIGO2 expression and representative signatures in TCGA pan-cancer dataset. Ns: no significant difference, * P < 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
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a Expression of <t>AMIGO2,</t> STC2, INHBA, DKK1, VEGFC, SPOCK1, MT1E, and FOXD1 in scRNA-seq UMAP plots. b Bar plot showing cell type proportions under LRS subtypes from scRNA-seq data. c AMIGO2, ranked highly for prognostic importance, was selected for further analysis. d AMIGO2 expression is upregulated in most tumor tissues across various cancers in TCGA pan-cancer dataset. e Evaluation of AMIGO2’s prognostic value (OS) in TCGA pan-cancer dataset. f Correlation analysis between AMIGO2 expression and representative signatures in TCGA pan-cancer dataset. Ns: no significant difference, * P < 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
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Image Search Results


Silencing of FKBP5 blunts dexamethasone (DEX)-regulated expression of glucocorticoid response element-containing genes in primary leiomyoma cell cultures. qPCR analysis of mRNA levels for DEX-upregulated genes TSC22D 3 , ADH1B, IL1R1 (A), and DEX-downregulated genes GREM1, IGFBP1, AMIGO2 (B) in cultured leiomyoma cells transfected with either control or FKBP5 siRNA for 48 hours and then treated with either vehicle (Cont) or DEX for 24 hours. Bars represent mean ± standard error of the mean, n = 4.

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Increased HSD11β1 Expression in Human Leiomyomatous Uteri: Implication for Enhanced Glucocorticoid Signaling

doi: 10.1210/clinem/dgaf255

Figure Lengend Snippet: Silencing of FKBP5 blunts dexamethasone (DEX)-regulated expression of glucocorticoid response element-containing genes in primary leiomyoma cell cultures. qPCR analysis of mRNA levels for DEX-upregulated genes TSC22D 3 , ADH1B, IL1R1 (A), and DEX-downregulated genes GREM1, IGFBP1, AMIGO2 (B) in cultured leiomyoma cells transfected with either control or FKBP5 siRNA for 48 hours and then treated with either vehicle (Cont) or DEX for 24 hours. Bars represent mean ± standard error of the mean, n = 4.

Article Snippet: The full name and Taq-Man Probe Assay IDs for each target gene include: FK506-binding protein 5 ( FKBP5 )- Hs01561006_m1; Hydroxysteroid 11-β dehydrogenase 1 ( HSD11B1) - Hs01547870_m1; IL-1 receptor type 1 ( IL1R1 )- Hs00991002_m1; TSC22 domain family member 3 ( TSC22D3 )- Hs00608272_m1; alcohol dehydrogenase 1B, β polypeptide ( ADH1B )- Hs00605175_m1; Gremlin 1, DAN family BMP antagonist ( GREM1 )- Hs00171951_m1; IGF-binding protein 5 ( IGFBP5 )- Hs00181213_m1; adhesion molecule with Ig-like domain 2 ( AMIGO2 )- Hs05001325_s1; laminin subunit alpha 2 ( LAMA2 )- Hs00166308_m1; fibronectin 1 ( FN1 )- Hs00365052_m1; calponin 1 ( CNN1 )- Hs00959434_m1; actin β ( ACTB )- Hs99999903_m1; and glyceraldehyde-3-phosphate dehydrogenase ( GAPDH )- Hs99999905_m1.

Techniques: Expressing, Cell Culture, Transfection, Control

a Expression of AMIGO2, STC2, INHBA, DKK1, VEGFC, SPOCK1, MT1E, and FOXD1 in scRNA-seq UMAP plots. b Bar plot showing cell type proportions under LRS subtypes from scRNA-seq data. c AMIGO2, ranked highly for prognostic importance, was selected for further analysis. d AMIGO2 expression is upregulated in most tumor tissues across various cancers in TCGA pan-cancer dataset. e Evaluation of AMIGO2’s prognostic value (OS) in TCGA pan-cancer dataset. f Correlation analysis between AMIGO2 expression and representative signatures in TCGA pan-cancer dataset. Ns: no significant difference, * P < 0.05, ** P ≤ 0.01, *** P ≤ 0.001.

Journal: NPJ Precision Oncology

Article Title: Metabolomic and transcriptomic profiling of HNSCC identifies AMIGO2 as a therapeutic target modulating tumor microenvironment

doi: 10.1038/s41698-025-01132-z

Figure Lengend Snippet: a Expression of AMIGO2, STC2, INHBA, DKK1, VEGFC, SPOCK1, MT1E, and FOXD1 in scRNA-seq UMAP plots. b Bar plot showing cell type proportions under LRS subtypes from scRNA-seq data. c AMIGO2, ranked highly for prognostic importance, was selected for further analysis. d AMIGO2 expression is upregulated in most tumor tissues across various cancers in TCGA pan-cancer dataset. e Evaluation of AMIGO2’s prognostic value (OS) in TCGA pan-cancer dataset. f Correlation analysis between AMIGO2 expression and representative signatures in TCGA pan-cancer dataset. Ns: no significant difference, * P < 0.05, ** P ≤ 0.01, *** P ≤ 0.001.

Article Snippet: The following primary antibodies were used for Western blotting: AMIGO2 (1:500, HUAAN, catalog No: ER1903-66), GAPDH (1:1000, CST, catalog No: 5174S, RRID: AB_10622025), E-cadherin (1:1000, CST, catalog No: 3195S, RRID: AB_2291471), N-cadherin (1:1000, CST, catalog No: 13116S, RRID: AB_2687616), HPRT1 (1:1000, Proteintech Cat# 15059-1-AP, RRID: AB_10638622), PAICS (1:1000, Proteintech Cat# 12967-1-AP, RRID: AB_10638449), Vimentin (1:1000, CST, catalog No: 5741S, RRID: AB_10695459), MMP9 (1:1000, CST, catalog No: 13667S, RRID: AB_2798289), FAP (Thermo Fisher Scientific Cat# PA5-99313, RRID: AB_2818246), α-SMA (Cell Signaling Technology Cat# 19245, RRID: AB_2734735), PI3K (Cell Signaling Technology Cat# 4292, RRID:AB_329869), Phospho-PI3K (Cell Signaling Technology Cat# 17366, RRID:AB_2895293), AKT (Cell Signaling Technology Cat# 9272, RRID: AB_329827), Phospho-Akt (Thr308) (Cell Signaling Technology Cat# 9275, RRID: AB_329828), Phospho-Akt (Ser473) (Cell Signaling Technology Cat# 9271, RRID: AB_329825), PDK1 (Thermo Fisher Scientific Cat# A302-130A, RRID:AB_1720395), Phospho-PDK1 (Ser241) (Cell Signaling Technology Cat# 3061, RRID: AB_2161919), mTOR (Abcam Cat# ab134903, RRID: AB_2800465) and mTOR (phospho S2481) (Abcam Cat# ab137133, RRID:AB_2800466).

Techniques: Expressing

a IHC images and quantitative statistics of AMIGO2 expression in adjacent normal tissue, precancerous lesions, and tumor tissues. b Representative images and quantification of CD8, CD56, FAP, and PanCK staining in tumor tissues from high-AMIGO2 and low-AMIGO2 patient groups. c Graphical abstract for this study. Ns: no significant difference. * P < 0.05, ** P ≤ 0.01, *** P ≤ 0.001.

Journal: NPJ Precision Oncology

Article Title: Metabolomic and transcriptomic profiling of HNSCC identifies AMIGO2 as a therapeutic target modulating tumor microenvironment

doi: 10.1038/s41698-025-01132-z

Figure Lengend Snippet: a IHC images and quantitative statistics of AMIGO2 expression in adjacent normal tissue, precancerous lesions, and tumor tissues. b Representative images and quantification of CD8, CD56, FAP, and PanCK staining in tumor tissues from high-AMIGO2 and low-AMIGO2 patient groups. c Graphical abstract for this study. Ns: no significant difference. * P < 0.05, ** P ≤ 0.01, *** P ≤ 0.001.

Article Snippet: The following primary antibodies were used for Western blotting: AMIGO2 (1:500, HUAAN, catalog No: ER1903-66), GAPDH (1:1000, CST, catalog No: 5174S, RRID: AB_10622025), E-cadherin (1:1000, CST, catalog No: 3195S, RRID: AB_2291471), N-cadherin (1:1000, CST, catalog No: 13116S, RRID: AB_2687616), HPRT1 (1:1000, Proteintech Cat# 15059-1-AP, RRID: AB_10638622), PAICS (1:1000, Proteintech Cat# 12967-1-AP, RRID: AB_10638449), Vimentin (1:1000, CST, catalog No: 5741S, RRID: AB_10695459), MMP9 (1:1000, CST, catalog No: 13667S, RRID: AB_2798289), FAP (Thermo Fisher Scientific Cat# PA5-99313, RRID: AB_2818246), α-SMA (Cell Signaling Technology Cat# 19245, RRID: AB_2734735), PI3K (Cell Signaling Technology Cat# 4292, RRID:AB_329869), Phospho-PI3K (Cell Signaling Technology Cat# 17366, RRID:AB_2895293), AKT (Cell Signaling Technology Cat# 9272, RRID: AB_329827), Phospho-Akt (Thr308) (Cell Signaling Technology Cat# 9275, RRID: AB_329828), Phospho-Akt (Ser473) (Cell Signaling Technology Cat# 9271, RRID: AB_329825), PDK1 (Thermo Fisher Scientific Cat# A302-130A, RRID:AB_1720395), Phospho-PDK1 (Ser241) (Cell Signaling Technology Cat# 3061, RRID: AB_2161919), mTOR (Abcam Cat# ab134903, RRID: AB_2800465) and mTOR (phospho S2481) (Abcam Cat# ab137133, RRID:AB_2800466).

Techniques: Expressing, Staining